Bovine leukocyte adhesion deficiency (BLAD) is a genetically transmitted disease and inherited as an autosomal recessive defect. Keeping this in view the present study was conducted to estimate incidence of carrier animals in a herd of breeding bulls for mutation in Integrin â2 gene, reared at Frozen Semen Bull Station (FSBS), Haringhata, Nadia, West Bengal, India. A total of 80 bulls of different age group were randomly selected from different cattle breeds (Sahiwal, Gir) to screen presence of BLAD syndrome in breeding sires. Genomic DNA was isolated from blood of the selected bulls. PCR parameters were standardized to obtain 570 bp amplicon. This amplified PCR product (amplicon) of ITGB2 gene was digested using TaqI (5’T“!CGA3’) restriction enzyme for screening of mutation in BLAD allele. TaqI PCR-RFLP revealed no such mutation thus indicating the absence of bovine leukocyte adhesion deficiency (BuLAD) allele in animals under study.
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